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(2003) MultiTag: multiple error-tolerant sequence tag search for the sequence-similarity identification of proteins by mass spectrometry. J., Golod A., Shevchenko A., Shevchenko A. (2003) GutenTag: high-throughput sequence tagging via an empirically derived fragmentation model. (2011) Implementation and uses of automated de novo peptide sequencing by tandem mass spectrometry. (1994) Error-tolerant identification of peptides in sequence databases by peptide sequence tags.
#DE NOVO PROTEIN SEQUENCE ANALYSIS SOFTWARE#
(2016) TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization. (2012) Protein identification using top-down spectra. Liu X., Sirotkin Y., Shen Y., Anderson G., Tsai Y.Nucleic Acids Res., 35(Web Server issue):W701-W706. (2007) ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry. Nucleic Acids Res., 32(Web Server issue):W340-W345. (2004) ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry. (2011) Andromeda: A peptide search engine integrated into the MaxQuant environment. Cox J., Neuhauser N., Michalski A., Scheltema R.(2014) MS-GF+ makes progress towards a universal database search tool for proteomics. (2008) Spectral probabilities and generating functions of tandem mass spectra: a strike against decoy databases. (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. (1994) An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. (1950) Method for determination of the amino acid sequence in peptides. (1949) A method for the determination of amino acid sequence in peptides. In this paper we briefly review the existing approaches to de novo sequencing of proteins and peptides, along with the problems that can be solved using those, and indicate directions and perspectives for their further development. The appeal of the latter is due to their applicability to investigating unknown proteins, as well as the ones that cannot be analyzed with genomics or transcriptomics methods. The results of mass spectrometric measurements can be interpreted by means of either database search or de novo sequencing methods. Currently, mass spectrometry is commonly applied to this end. Mass spectrometry de novo sequencing proteins peptides amino acid sequence AbstractÄetermination of the primary structure of proteins and peptides constitutes an important step in studying their properties. Saint Petersburg National Research Academic University of the Russian Academy of Sciences 8/3 Khlopina st., St Petersburg 194021, Russia Saint Petersburg State University 7-9 Universitetskaya nab., St Petersburg, 199034 Russia